lambda dg4 150 watt xenon light source Search Results


150 w xenon lamp  (Sutter Instrument)
90
Sutter Instrument 150 w xenon lamp
150 W Xenon Lamp, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda dg4 150 watt xenon light source  (Sutter Instrument Company)
90
Sutter Instrument Company lambda dg4 150 watt xenon light source
Lambda Dg4 150 Watt Xenon Light Source, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda dg4 150 watt xenon light source/product/Sutter Instrument Company
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150 w xenon arc lamp lambda dg-4  (Sutter Instrument Company)
90
Sutter Instrument Company 150 w xenon arc lamp lambda dg-4
150 W Xenon Arc Lamp Lambda Dg 4, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda dg-4 150 watt xenon light source  (Sutter Instrument)
90
Sutter Instrument lambda dg-4 150 watt xenon light source
Lambda Dg 4 150 Watt Xenon Light Source, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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emccd camera  (Oxford Instruments)
90
Oxford Instruments emccd camera
Emccd Camera, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c9100-02 em ccd camera  (Hamamatsu)
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Hamamatsu c9100-02 em ccd camera
C9100 02 Em Ccd Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluo-4 am  (Thermo Fisher)
90
Thermo Fisher fluo-4 am
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Fluo 4 Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverted microscope diaphot 200  (Nikon)
90
Nikon inverted microscope diaphot 200
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Inverted Microscope Diaphot 200, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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filter set 31001  (Chroma Technology Corporation)
90
Chroma Technology Corporation filter set 31001
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Filter Set 31001, supplied by Chroma Technology Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microscope  (Nikon)
96
Nikon microscope
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert fluorescence microscope  (Carl Zeiss)
90
Carl Zeiss axiovert fluorescence microscope
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Axiovert Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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epifluorescence te 200 inverted microscope  (Nikon)
90
Nikon epifluorescence te 200 inverted microscope
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Epifluorescence Te 200 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epifluorescence te 200 inverted microscope/product/Nikon
Average 90 stars, based on 1 article reviews
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Image Search Results


The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with Fluo-4 and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).

Journal: American Journal of Physiology - Cell Physiology

Article Title: Intracellular pH modulates inner segment calcium homeostasis in vertebrate photoreceptors

doi: 10.1152/ajpcell.00264.2010

Figure Lengend Snippet: The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with Fluo-4 and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).

Article Snippet: For uncaging experiments, cells were incubated simultaneously with 20 μM of nitrophenyl (NP)-EGTA AM and 5 μM Fluo-4 AM (Invitrogen) for 20 min; Ca 2+ was uncaged by 5-ms flashes of white light (340–700 nm broadband excitation at 150 W, Lambda DG4; Sutter Instruments) delivered via the liquid light guide from the Xenon arc lamp.

Techniques: